PDBe 1fak

X-ray diffraction
2.1Å resolution

HUMAN TISSUE FACTOR COMPLEXED WITH COAGULATION FACTOR VIIA INHIBITED WITH A BPTI-MUTANT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 152 amino acids
Theoretical weight: 17.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 61-212; Coverage: 34%)
Gene name: F7
Sequence domains:
Structure domains: Laminin
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 206 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 37-242; Coverage: 78%)
Gene name: F3
Structure domains: Immunoglobulins
Pancreatic trypsin inhibitor Chain: I
Molecule details ›
Chain: I
Length: 55 amino acids
Theoretical weight: 6.3 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00974 (Residues: 37-90; Coverage: 68%)
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 63.49Å b: 190Å c: 175.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 not available not available
Expression systems:
  • Cricetulus griseus
  • Escherichia coli
  • Not provided