1fa6

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE CO(II)-BOUND GLYOXALASE I OF ESCHERICHIA COLI

Released:

Function and Biology Details

Reaction catalysed:
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC81 (Residues: 1-135; Coverage: 100%)
Gene names: JW1643, b1651, gloA
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 46.09Å b: 56.57Å c: 46.75Å
α: 90° β: 95.54° γ: 90°
R-values:
R R work R free
0.175 0.169 0.254
Expression system: Escherichia coli