› 1f9g

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE COCRYSTALLIZED WITH ASCORBIC ACID

Released:
DOI: 10.2210/pdb1f9g/pdb
PDB 1f9g coloured by chain and viewed from the front.
PDB 1f9g coloured by chain and viewed from the side.
PDB 1f9g coloured by chain and viewed from the top.
Source organism: Streptococcus pneumoniae
Primary publication:
Vitamin C inhibits the enzymatic activity of Streptococcus pneumoniae hyaluronate lyase.
Li S, Taylor KB, Kelly SJ, Jedrzejas MJ
J Biol Chem 276 15125-30 (2001)
PMID: 11278838

Function and Biology Details

Reaction catalysed: 
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hyaluronate lyase Chain: A
Molecule details ›
Chain: A
Length: 731 amino acids
Theoretical weight: 83.56 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54873 (Residues: 285-1009; Coverage: 70%)
Gene name: SP_0314
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ASC 1 x ASC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 84.264Å b: 102.666Å c: 103.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.252
Expression system: Escherichia coli

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Citations

3 review citations

Paving the way towards effective plant-based inhibitors of hyaluronidase and tyrosinase: a critical review on a structure-activity relationship.
Gębalski et al. (2022)
2 more

3 mentions without citation

Automated identification of protein-ligand interaction features using Inductive Logic Programming: a hexose binding case study.
A Santos et al. (2012)
2 more