1f8i

X-ray diffraction
2.25Å resolution

CRYSTAL STRUCTURE OF ISOCITRATE LYASE:NITROPROPIONATE:GLYOXYLATE COMPLEX FROM MYCOBACTERIUM TUBERCULOSIS

Released:

Function and Biology Details

Reaction catalysed:
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 429 amino acids
Theoretical weight: 47.19 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKK7 (Residues: 2-428; Coverage: 100%)
Gene names: MTV038.11, Rv0467, icl
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 74.291Å b: 129.018Å c: 166.962Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.166 0.21
Expression system: Escherichia coli