1f80

X-ray diffraction
2.3Å resolution

HOLO-(ACYL CARRIER PROTEIN) SYNTHASE IN COMPLEX WITH HOLO-(ACYL CARRIER PROTEIN)

Released:

Function and Biology Details

Reaction catalysed:
CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Holo-[acyl-carrier-protein] synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 120 amino acids
Theoretical weight: 13.51 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P96618 (Residues: 2-121; Coverage: 99%)
Gene names: BSU04620, acpS, ydcB
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain
Acyl carrier protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 81 amino acids
Theoretical weight: 9.2 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P80643 (Residues: 2-77; Coverage: 99%)
Gene names: BSU15920, acpA, acpP
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2221
Unit cell:
a: 78.458Å b: 122.032Å c: 136.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.222 0.279
Expression system: Escherichia coli