1f5z

X-ray diffraction
1.88Å resolution

CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM I

Released:

Function and Biology Details

Reaction catalysed:
N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 32.56 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44539 (Residues: 1-293; Coverage: 100%)
Gene names: HI_0142, nanA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 84.753Å b: 109.778Å c: 133.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.246
Expression system: Escherichia coli