1f5t

X-ray diffraction
3Å resolution

DIPHTHERIA TOX REPRESSOR (C102D MUTANT) COMPLEXED WITH NICKEL AND DTXR CONSENSUS BINDING SEQUENCE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Diphtheria toxin repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 13.92 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJL7 (Residues: 1-121; Coverage: 54%)
Gene names: DIP1414, dtxR
Sequence domains: Iron dependent repressor, N-terminal DNA binding domain
Structure domains:
43MER DNA CONTAINING DXTR CONSENSUS BINDING SEQUENCE Chain: E
Molecule details ›
Chain: E
Length: 43 nucleotides
Theoretical weight: 13.22 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Not provided
43MER DNA CONTAINING DXTR CONSENSUS BINDING SEQUENCE Chain: F
Molecule details ›
Chain: F
Length: 43 nucleotides
Theoretical weight: 13.26 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41
Unit cell:
a: 116.178Å b: 116.178Å c: 142.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.273
Expression systems:
  • Escherichia coli
  • Not provided