1f26

X-ray diffraction
1.4Å resolution

CRYSTAL STRUCTURE OF NO COMPLEX OF THR243VAL MUTANTS OF CYTOCHROME P450NOR

Released:

Function and Biology Details

Reaction catalysed:
Nitrous oxide + NAD(P)(+) + H(2)O = 2 nitric oxide + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP nitrous oxide-forming nitric oxide reductase Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 44.29 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P23295 (Residues: 2-403; Coverage: 100%)
Gene names: CYP55, CYP55A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 54.567Å b: 81.938Å c: 85.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.139 0.207
Expression system: Escherichia coli K-12