1f0i

X-ray diffraction
1.4Å resolution

THE FIRST CRYSTAL STRUCTURE OF A PHOSPHOLIPASE D

Released:
Source organism: Streptomyces sp. PMF
Primary publication:
The first crystal structure of a phospholipase D.
Structure 8 655-67 (2000)
PMID: 10873862

Function and Biology Details

Reaction catalysed:
A phosphatidylcholine + H(2)O = choline + a phosphatidate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase D Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 53.72 KDa
Source organism: Streptomyces sp. PMF
UniProt:
  • Canonical: P84147 (Residues: 3-506; Coverage: 100%)
Sequence domains: PLD-like domain
Structure domains: Endonuclease Chain A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P21
Unit cell:
a: 57.28Å b: 57.42Å c: 68.7Å
α: 90° β: 93.17° γ: 90°
R-values:
R R work R free
0.13 0.13 0.185