› 1ewo

X-ray diffraction
2.1Å resolution

THE CYSTEINE PROTEASE CRUZAIN BOUND TO WRR-204

Released:
DOI: 10.2210/pdb1ewo/pdb
PDB entry 1ewo coloured by chain, front view.
PDB entry 1ewo coloured by chain, side view.
PDB entry 1ewo coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Crystal Structure of Cruzain bound to WRR-204
Brinen LS, Gillmor SA, Fletterick RJ
To be published

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity similar to that of cathepsin L.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cruzipain Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 22.72 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: P25779 (Residues: 123-337; Coverage: 48%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand VSC 1 x VSC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 45.206Å b: 51.551Å c: 45.511Å
α: 90° β: 116.13° γ: 90°
R-values:
R R work R free
0.23 0.221 0.262
Expression system: Escherichia coli

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