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X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 COMPLEXED WITH GLUTAMATE

Released:
DOI: 10.2210/pdb1ewk/pdb
PDB 1ewk coloured by chain and viewed from the front.
PDB 1ewk coloured by chain and viewed from the side.
PDB 1ewk coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor.
Kunishima N, Shimada Y, Tsuji Y, Sato T, Yamamoto M, Kumasaka T, Nakanishi S, Jingami H, Morikawa K
Nature 407 971-7 (2000)
PMID: 11069170

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metabotropic glutamate receptor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 55.26 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23385 (Residues: 33-522; Coverage: 42%)
Gene names: Gprc1a, Grm1, Mglur1
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand MG 2 x MG
Ligand GLU 2 x GLU
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 83.375Å b: 95.219Å c: 97.451Å
α: 90° β: 114.85° γ: 90°
R-values:
R R work R free
0.198 0.196 0.227
Expression system: Spodoptera frugiperda

Quick links

Citations

189 review citations

Allosteric Molecular Switches in Metabotropic Glutamate Receptors.
Orgován et al. (2021)
188 more

64 mentions without citation

Structural biology of GABAB receptor.
Frangaj et al. (2018)
63 more