› 1ev9

X-ray diffraction
2.2Å resolution

RAT GLUTATHIONE S-TRANSFERASE A1-1 MUTANT W21F WITH GSO3 BOUND

Released:
DOI: 10.2210/pdb1ev9/pdb
PDB entry 1ev9 coloured by chain, front view.
PDB entry 1ev9 coloured by chain, side view.
PDB entry 1ev9 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Localization of the C-terminus of rat glutathione S-transferase A1-1: crystal structure of mutants W21F and W21F/F220Y.
Adman ET, Le Trong I, Stenkamp RE, Nieslanik BS, Dietze EC, Tai G, Ibarra C, Atkins WM
Proteins 42 192-200 (2001)
PMID: 11119643

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase alpha-1 Chains: A, C, D
Molecule details ›
Chains: A, C, D
Length: 221 amino acids
Theoretical weight: 25.46 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00502 (Residues: 2-222; Coverage: 100%)
Gene name: Gsta1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTS 3 x GTS
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 84.7Å b: 275.1Å c: 70.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.299
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
2 more

2 mentions without citation

Glutathione transferases are structural and functional outliers in the thioredoxin fold.
Atkinson et al. (2009)
1 more