1ev7

X-ray diffraction
2.38Å resolution

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 restriction enzyme NaeI Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.38 KDa
Source organism: Lechevalieria aerocolonigenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P50187 (Residues: 1-317; Coverage: 100%)
Gene name: naeIR
Sequence domains: Restriction endonuclease NaeI
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 99.78Å b: 56.16Å c: 59.05Å
α: 90° β: 95.92° γ: 90°
R-values:
R R work R free
0.238 0.238 0.287
Expression system: Escherichia coli