› 1et4

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF A VITAMIN B12 BINDING RNA APTAMER WITH LIGAND AT 2.3 A

Released:
DOI: 10.2210/pdb1et4/pdb
PDB entry 1et4 coloured by chain, front view.
PDB entry 1et4 coloured by chain, side view.
PDB entry 1et4 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
A water channel in the core of the vitamin B(12) RNA aptamer.
Sussman D, Wilson C
Structure 8 719-27 (2000)
PMID: 10903943

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196007 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA APTAMER, 35-MER Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 35 nucleotides
Theoretical weight: 11.28 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CNC 5 x CNC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I222
Unit cell:
a: 91.27Å b: 98.08Å c: 217.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.232
Expression system: Not provided

Quick links

Citations

3 review citations

RNA-based therapeutics: current progress and future prospects.
Burnett et al. (2012)
2 more

8 mentions without citation

Selection and Biosensor Application of Aptamers for Small Molecules.
Pfeiffer et al. (2016)
7 more