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E.COLI PRIMASE CATALYTIC CORE

Source organism: Escherichia coli
Biochemical function: nucleotidyltransferase activity
Biological process: DNA replication, synthesis of primer
Cellular component: not assigned

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EC 2.7.7.101: DNA primase DnaG

Reaction catalysed:
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Systematic name:
Nucleotide 5'-triphosphate:single-stranded DNA nucleotidyltransferase (DNA-RNA hybrid synthesizing)
Alternative Name(s):
  • DnaG

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF10410
Domain description: DnaB-helicase binding domain of primase
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of Pfam domain PF10410 (DnaB-helicase binding domain of primase) in DNA primase. Showing 1 copy in chain A.

PF13155
Domain description: Toprim-like
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of Pfam domain PF13155 (Toprim-like) in DNA primase. Showing 1 copy in chain A.

PF08275
Domain description: DNA primase catalytic core, N-terminal domain
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of Pfam domain PF08275 (DNA primase catalytic core, N-terminal domain) in DNA primase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR006171
Domain description: TOPRIM domain
Occurring in:
  1. DNA primase
IPR019475
Domain description: DNA primase, DnaB-helicase binding domain
Occurring in:
  1. DNA primase
IPR034151
Domain description: Bacterial DnaG primase, TOPRIM domain
Occurring in:
  1. DNA primase
IPR013264
Domain description: DNA primase DNAG, catalytic core, N-terminal
Occurring in:
  1. DNA primase
IPR037068
Domain description: DNA primase, catalytic core, N-terminal domain superfamily
Occurring in:
  1. DNA primase
IPR006295
Domain description: DNA primase, DnaG
Occurring in:
  1. DNA primase

Structure domains

CATH CATH domains
1.20.50.20
Class: Mainly Alpha
Architecture: Up-down Bundle
Topology: Pheromone ER-1
Homology: DnaG, RNA polymerase domain, helical bundle
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of CATH domain 1.20.50.20 (Pheromone ER-1) in DNA primase. Showing 1 copy in chain A.
3.40.1360.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Dna Topoisomerase Vi A Subunit; Chain: A, domain 2
Homology: Dna Topoisomerase Vi A Subunit; Chain: A, domain 2
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of CATH domain 3.40.1360.10 (Dna Topoisomerase Vi A Subunit; Chain: A, domain 2) in DNA primase. Showing 1 copy in chain A.
3.90.980.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: DNA primase DNAg catalytic core, N-terminal domain
Homology: DNA primase, catalytic core, N-terminal domain
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of CATH domain 3.90.980.10 (DNA primase DNAg catalytic core, N-terminal domain) in DNA primase. Showing 1 copy in chain A.
SCOP SCOP annotation
56732
Class: Multi-domain proteins (alpha and beta)
Fold: DNA primase core
Superfamily: DNA primase core
Occurring in:
  1. DNA primase
The deposited structure of PDB entry 1eqn contains 5 copies of SCOP domain 56732 (DNA primase DnaG catalytic core) in DNA primase. Showing 1 copy in chain A.

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