› 1eqf

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE DOUBLE BROMODOMAIN MODULE FROM HUMAN TAFII250

Released:
DOI: 10.2210/pdb1eqf/pdb
PDB 1eqf coloured by chain and viewed from the front.
PDB 1eqf coloured by chain and viewed from the side.
PDB 1eqf coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structure and function of a human TAFII250 double bromodomain module.
Jacobson RH, Ladurner AG, King DS, Tjian R
Science 288 1422-5 (2000)
PMID: 10827952

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor TFIID subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 32.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21675 (Residues: 1359-1638; Coverage: null%)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 48.1Å b: 58.1Å c: 101.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.218 0.246
Expression system: Escherichia coli

Quick links

Citations

165 review citations

Recent insights into the structure of TFIID, its assembly, and its binding to core promoter.
Patel et al. (2020)
164 more

15 mentions without citation

Zooming in on Transcription Preinitiation.
Gupta et al. (2016)
14 more