› 1eqd

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF NITROPHORIN 4 COMPLEXED WITH CN

Released:
DOI: 10.2210/pdb1eqd/pdb
PDB entry 1eqd coloured by chain, front view.
PDB entry 1eqd coloured by chain, side view.
PDB entry 1eqd coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial.
Weichsel A, Andersen JF, Roberts SA, Montfort WR
Nat Struct Biol 7 551-4 (2000)
PMID: 10876239

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand CYN 1 x CYN
Ligand HEV 1 x HEV
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2
Unit cell:
a: 70.036Å b: 42.652Å c: 52.559Å
α: 90° β: 94.18° γ: 90°
R-values:
R R work R free
0.2 0.2 0.26

Quick links

Citations

11 review citations

Neutrophil gelatinase-associated lipocalin-mediated iron traffic in kidney epithelia.
Schmidt-Ott et al. (2006)
10 more

5 mentions without citation

New light on NO bonding in Fe(III) heme proteins from resonance Raman spectroscopy and DFT modeling.
Soldatova et al. (2010)
4 more