1ep2

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE B COMPLEXED WITH OROTATE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 32.98 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P54322 (Residues: 1-311; Coverage: 100%)
Gene names: llmg_1106, pyrDB
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I
Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 28.56 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P56968 (Residues: 2-262; Coverage: 100%)
Gene names: llmg_1105, pyrK
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN

Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R32
Unit cell:
a: 202.42Å b: 202.42Å c: 80.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.241
Expression system: Escherichia coli