› 1elj

X-ray diffraction
1.85Å resolution

THE CRYSTAL STRUCTURE OF LIGANDED MALTODEXTRIN-BINDING PROTEIN FROM PYROCOCCUS FURIOSUS

Released:
DOI: 10.2210/pdb1elj/pdb
PDB entry 1elj coloured by chain, front view.
PDB entry 1elj coloured by chain, side view.
PDB entry 1elj coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein.
Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS
J Mol Biol 305 891-904 (2001)
PMID: 11162100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltotriose-binding protein Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 43.15 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58300 (Residues: 59-434; Coverage: 91%)
Gene names: PF1938, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 61.118Å b: 68.832Å c: 127.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.229
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Geometry of nonbonded interactions involving planar groups in proteins.
Chakrabarti et al. (2007)
3 more

4 mentions without citation

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217.
Wada et al. (2007)
3 more