› 1ek8

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1ek8/pdb
PDB entry 1ek8 coloured by chain, front view.
PDB entry 1ek8 coloured by chain, side view.
PDB entry 1ek8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the ribosome recycling factor from Escherichia coli.
Kim KK, Min K, Suh SW
EMBO J 19 2362-70 (2000)
PMID: 10811627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-recycling factor Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A805 (Residues: 1-185; Coverage: 100%)
Gene names: JW0167, b0172, frr, rrf
Sequence domains: Ribosome recycling factor
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HG 3 x HG
Ligand DEM 1 x DEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 48.064Å b: 48.064Å c: 142.273Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.228 0.297
Expression system: Escherichia coli

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Citations

4 review citations

Translational termination comes of age.
Kisselev et al. (2000)
3 more

10 mentions without citation

Aligator: A computational tool for optimizing total chemical synthesis of large proteins.
Jacobsen et al. (2017)
9 more