› 1eiz

X-ray diffraction
1.7Å resolution

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE

Released:
DOI: 10.2210/pdb1eiz/pdb
PDB entry 1eiz coloured by chain, front view.
PDB entry 1eiz coloured by chain, side view.
PDB entry 1eiz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
RNA methylation under heat shock control.
Bügl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U
Mol Cell 6 349-60 (2000)
PMID: 10983982

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase E Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 19.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C0R7 (Residues: 30-209; Coverage: 86%)
Gene names: JW3146, b3179, ftsJ, mrsF, rlmE, rrmJ
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 36.457Å b: 65.867Å c: 72.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

The heat shock response: life on the verge of death.
Richter et al. (2010)
28 more

15 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
14 more