Function and Biology Details
Reaction catalysed:
5,10-methylenetetrahydrofolate + NAD(+) = 5,10-methenyltetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Tetrahydrofolate dehydrogenase/cyclohydrolase
- NAD(P)-binding domain superfamily
- Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
- Methylenetetrahydrofolate dehydrogenase, NAD-binding domain
- Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
- Aminoacid dehydrogenase-like, N-terminal domain superfamily
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Methylenetetrahydrofolate dehydrogenase [NAD(+)] (preferred)
PDBe Complex ID:
PDB-CPX-169660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylenetetrahydrofolate dehydrogenase [NAD(+)]
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 36.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 320 amino acids
Theoretical weight: 36.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
- Canonical:
Q02046 (Residues: 1-320; Coverage: 100%)
Sequence domains:
- Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
- Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU RU200
Spacegroup:
P41212
Expression system: Escherichia coli
