1eay

X-ray diffraction
2Å resolution

CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Chemotaxis protein CheA Chains: C, D
Molecule details ›
Chains: C, D
Length: 74 amino acids
Theoretical weight: 8.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P07363 (Residues: 156-228; Coverage: 11%)
Gene names: JW1877, b1888, cheA
Sequence domains: CheY binding
Structure domains: CheY-binding domain of CheA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 54.5Å b: 64.2Å c: 158Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 not available