› 1eab

X-ray diffraction
2.6Å resolution

ATOMIC STRUCTURE OF THE CUBIC CORE OF THE PYRUVATE DEHYDROGENASE MULTIENZYME COMPLEX

Released:
DOI: 10.2210/pdb1eab/pdb
PDB 1eab coloured by chain and viewed from the front.
PDB 1eab coloured by chain and viewed from the side.
PDB 1eab coloured by chain and viewed from the top.
Source organism: Azotobacter vinelandii
Primary publication:
Crystallographic analysis of substrate binding and catalysis in dihydrolipoyl transacetylase (E2p).
Mattevi A, Obmolova G, Kalk KH, Teplyakov A, Hol WG
Biochemistry 32 3887-901 (1993)
PMID: 8471601

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 26.24 KDa
Source organism: Azotobacter vinelandii
Expression system: Not provided
UniProt:
  • Canonical: P10802 (Residues: 396-638; Coverage: 38%)
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments


Cofactor: Ligand COA 1 x COA
1 bound ligand:
Ligand LPM 1 x LPM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: F432
Unit cell:
a: 225.54Å b: 225.54Å c: 225.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 not available
Expression system: Not provided

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Citations

7 review citations

Protein cages and synthetic polymers: a fruitful symbiosis for drug delivery applications, bionanotechnology and materials science.
Rother et al. (2016)
6 more

1 mention without citation

Recurrent Germline DLST Mutations in Individuals with Multiple Pheochromocytomas and Paragangliomas.
Remacha et al. (2019)