1e8o

X-ray diffraction
3.2Å resolution

Core of the Alu domain of the mammalian SRP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 9 kDa protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 85 amino acids
Theoretical weight: 10 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49458 (Residues: 2-86; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
Signal recognition particle 14 kDa protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 12.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37108 (Residues: 2-107; Coverage: 78%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1
7SL RNA Chain: E
Molecule details ›
Chain: E
Length: 50 nucleotides
Theoretical weight: 16.28 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 57.448Å b: 186.621Å c: 189.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.291
Expression system: Escherichia coli