1e73

X-ray diffraction
1.5Å resolution

2-F-glucosylated MYROSINASE FROM SINAPIS ALBA with bound L-ascorbate

Released:

Function and Biology Details

Reaction catalysed:
A thioglucoside + H(2)O = a sugar + a thiol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Myrosinase MA1 Chain: M
Molecule details ›
Chain: M
Length: 501 amino acids
Theoretical weight: 57.08 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: P29736 (Residues: 1-501; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC, MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 135.3Å b: 137.2Å c: 80.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.134 0.167