› 1e3p

X-ray diffraction
2.5Å resolution

tungstate derivative of Streptomyces antibioticus PNPase/GPSI enzyme

Released:
DOI: 10.2210/pdb1e3p/pdb
PDB 1e3p coloured by chain and viewed from the front.
PDB 1e3p coloured by chain and viewed from the side.
PDB 1e3p coloured by chain and viewed from the top.
Source organism: Streptomyces antibioticus
Primary publication:
A duplicated fold is the structural basis for polynucleotide phosphorylase catalytic activity, processivity, and regulation.
Symmons MF, Jones GH, Luisi BF
Structure 8 1215-26 (2000)
PMID: 11080643

Function and Biology Details

Reaction catalysed: 
RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyribonucleotide nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 757 amino acids
Theoretical weight: 81.23 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53597 (Residues: 1-740; Coverage: 100%)
Gene name: pnp
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand WO4 1 x WO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MSC
Spacegroup: R32
Unit cell:
a: 130.83Å b: 130.83Å c: 328.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.247
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase.
Falchi et al. (2022)
33 more

14 mentions without citation

Ro60 and Y RNAs: structure, functions, and roles in autoimmunity.
Boccitto et al. (2019)
13 more