› 1e1k

X-ray diffraction
1.95Å resolution

ADRENODOXIN REDUCTASE in complex with NADP+ obtained by a soaking experiment

Released:
DOI: 10.2210/pdb1e1k/pdb
PDB 1e1k coloured by chain and viewed from the front.
PDB 1e1k coloured by chain and viewed from the side.
PDB 1e1k coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
Ziegler GA, Schulz GE
Biochemistry 39 10986-95 (2000)
PMID: 10998235

Function and Biology Details

Reaction catalysed: 
2 reduced adrenodoxin + NADP(+) = 2 oxidized adrenodoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH:adrenodoxin oxidoreductase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 50.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08165 (Residues: 33-492; Coverage: 94%)
Gene names: ADXR, FDXR
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP

Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 60.9Å b: 62.4Å c: 78.2Å
α: 90° β: 106.6° γ: 90°
R-values:
R R work R free
0.184 0.184 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Mechanisms of Mitochondrial Redox Signaling in Psychosocial Stress-Responsive Systems: New Insights into an Old Story.
Meyer et al. (2018)
8 more