X-ray diffraction
2Å resolution

Subtilisin BPN' from Bacillus amyloliquefaciens, crystal growth mutant


Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Subtilisin BPN' Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 26.65 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Bacillus amyloliquefaciens
  • Canonical: P00782 (Residues: 108-382; Coverage: 76%)
Gene name: apr
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 46.289Å b: 58.527Å c: 85.168Å
α: 90° β: 90° γ: 90°
R R work R free
0.2 0.2 0.283
Expression system: Bacillus amyloliquefaciens