› 1dt6

X-ray diffraction
3Å resolution

STRUCTURE OF MAMMALIAN CYTOCHROME P450 2C5

Released:
DOI: 10.2210/pdb1dt6/pdb
PDB entry 1dt6 coloured by chain, front view.
PDB entry 1dt6 coloured by chain, side view.
PDB entry 1dt6 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversity.
Williams PA, Cosme J, Sridhar V, Johnson EF, McRee DE
Mol Cell 5 121-31 (2000)
PMID: 10678174

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2C5 Chain: A
Molecule details ›
Chain: A
Length: 473 amino acids
Theoretical weight: 53.88 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00179 (Residues: 21-487; Coverage: 96%)
Gene name: CYP2C5
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand SM 1 x SM
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 74.7Å b: 132Å c: 172.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.238 0.313
Expression system: Escherichia coli

Quick links

Citations

94 review citations

Aromatase--a brief overview.
Simpson et al. (2002)
93 more

24 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
23 more