› 1dr2

X-ray diffraction
2.3Å resolution

2.3 ANGSTROMS CRYSTAL STRUCTURE OF CHICKEN LIVER DIHYDROFOLATE REDUCTASE COMPLEXED WITH THIONADP+ AND BIOPTERIN

Released:
DOI: 10.2210/pdb1dr2/pdb
PDB entry 1dr2 coloured by chain, front view.
PDB entry 1dr2 coloured by chain, side view.
PDB entry 1dr2 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystal structures of chicken liver dihydrofolate reductase: binary thioNADP+ and ternary thioNADP+.biopterin complexes.
McTigue MA, Davies JF, Kaufman BT, Kraut J
Biochemistry 32 6855-62 (1993)
PMID: 8334118

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.68 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00378 (Residues: 1-189; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand TAP 1 x TAP
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 89.02Å b: 48.24Å c: 64.11Å
α: 90° β: 124.63° γ: 90°
R-values:
R R work R free
0.158 not available not available
Expression system: Not provided

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Citations

11 citation in other articles

Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center.
Raman et al. (1998)
10 more