› 1do2

X-ray diffraction
4Å resolution

TRIGONAL CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1do2/pdb
PDB 1do2 coloured by chain and viewed from the front.
PDB 1do2 coloured by chain and viewed from the side.
PDB 1do2 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
The structures of HsIU and the ATP-dependent protease HsIU-HsIV.
Bochtler M, Hartmann C, Song HK, Bourenkov GP, Bartunik HD, Huber R
Nature 403 800-5 (2000)
PMID: 10693812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease ATPase subunit HslU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 442 amino acids
Theoretical weight: 49.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6H5 (Residues: 2-443; Coverage: 100%)
Gene names: JW3902, b3931, hslU, htpI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P321
Unit cell:
a: 201.777Å b: 201.777Å c: 171.628Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

67 review citations

Assessing heterogeneity in oligomeric AAA+ machines.
Sysoeva TA. (2017)
66 more

9 mentions without citation

Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).
Zhao et al. (2012)
8 more