› 1dlf

X-ray diffraction
1.45Å resolution

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25

Released:
DOI: 10.2210/pdb1dlf/pdb
PDB entry 1dlf coloured by chain, front view.
PDB entry 1dlf coloured by chain, side view.
PDB entry 1dlf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The pH-dependent structural variation of complementarity-determining region H3 in the crystal structures of the Fv fragment from an anti-dansyl monoclonal antibody.
Nakasako M, Takahashi H, Shimba N, Shimada I, Arata Y
J Mol Biol 291 117-34 (1999)
PMID: 10438610

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224963 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig kappa chain V-II region 26-10 Chain: L
Molecule details ›
Chain: L
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01631 (Residues: 1-113; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
ANTI-DANSYL IMMUNOGLOBULIN IGG2A(S) Chain: H
Molecule details ›
Chain: H
Length: 124 amino acids
Theoretical weight: 14.15 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P212121
Unit cell:
a: 56.101Å b: 70.722Å c: 53.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.23

Quick links

Citations

1 review citation

Approaches to efficiently estimate solvation and explicit water energetics in ligand binding: the use of WaterMap.
Yang et al. (2013)
8 other articles

12 mentions without citation

Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.
Taylor et al. (2019)
11 more