› 1dju

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF AROMATIC AMINOTRANSFERASE FROM PYROCOCCUS HORIKOSHII OT3

Released:
DOI: 10.2210/pdb1dju/pdb
PDB entry 1dju coloured by chain, front view.
PDB entry 1dju coloured by chain, side view.
PDB entry 1dju coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii.
Matsui I, Matsui E, Sakai Y, Kikuchi H, Kawarabayasi Y, Ura H, Kawaguchi S, Kuramitsu S, Harata K
J Biol Chem 275 4871-9 (2000)
PMID: 10671523

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.84 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59096 (Residues: 2-389; Coverage: 100%)
Gene name: PH1371
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PLP 2 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P212121
Unit cell:
a: 64.01Å b: 124.87Å c: 128.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.254
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
4 more

5 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
4 more