1dfq

X-ray diffraction
2.6Å resolution

THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH SIALIC ACID

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 50.84 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli
UniProt:
  • Canonical: P04958 (Residues: 872-1315; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 66.86Å b: 70.24Å c: 122.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.313
Expression system: Escherichia coli