› 1det

X-ray diffraction
1.8Å resolution

RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP

Released:
DOI: 10.2210/pdb1det/pdb
PDB entry 1det coloured by chain, front view.
PDB entry 1det coloured by chain, side view.
PDB entry 1det coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP.
Ishikawa K, Suzuki E, Tanokura M, Takahashi K
Biochemistry 35 8329-34 (1996)
PMID: 8679590

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.15 KDa
Source organism: Aspergillus oryzae
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand 2GP 1 x 2GP
1 modified residue:
Ligand CGA 1 x CGA

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I23
Unit cell:
a: 88.69Å b: 88.69Å c: 88.69Å
α: 90° β: 90° γ: 90°

Quick links

Citations

2 review citations

A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.
Steyaert J. (1997)
1 more

3 mentions without citation

Structure and function studies on enzymes with a catalytic carboxyl group(s): from ribonuclease T1 to carboxyl peptidases.
Takahashi K. (2013)
2 more