› 1dd9

X-ray diffraction
1.6Å resolution

STRUCTURE OF THE DNAG CATALYTIC CORE

Released:
DOI: 10.2210/pdb1dd9/pdb
PDB entry 1dd9 coloured by chain, front view.
PDB entry 1dd9 coloured by chain, side view.
PDB entry 1dd9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the RNA polymerase domain of E. coli primase.
Keck JL, Roche DD, Lynch AS, Berger JM
Science 287 2482-6 (2000)
PMID: 10741967

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 38.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABS5 (Residues: 111-433; Coverage: 56%)
Gene names: JW3038, b3066, dnaG, dnaP, parB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SR 1 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 38.34Å b: 57.87Å c: 148.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.276
Expression system: Escherichia coli

Quick links

Citations

21 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
20 more

12 mentions without citation

The bacterial helicase-primase interaction: a common structural/functional module.
Soultanas P. (2005)
11 more