1dct

X-ray diffraction
2.8Å resolution

DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Modification methylase HaeIII Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Haemophilus influenzae biotype aegyptius
UniProt:
  • Canonical: P20589 (Residues: 1-324; Coverage: 98%)
Gene name: haeIIIM
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:
DNA (5'-D(*AP*CP*CP*AP*GP*CP*AP*GP*GP*(C49)P*CP*AP*CP*CP*AP*GP*TP*G)-3') Chains: F, G
Molecule details ›
Chains: F, G
Length: 18 nucleotides
Theoretical weight: 5.56 KDa
Source organism: Haemophilus influenzae biotype aegyptius
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*CP*TP*GP*GP*TP*GP*GP*(C5M)P*CP*TP*GP*CP*TP*GP*G)-3') Chains: M, N
Molecule details ›
Chains: M, N
Length: 18 nucleotides
Theoretical weight: 5.55 KDa
Source organism: Haemophilus influenzae biotype aegyptius
Expression system: Not provided

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 57.57Å b: 108.04Å c: 155.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.326
Expression system: Not provided