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Solution NMR

SOLUTION STRUCTURE OF THE TYPE I DOCKERIN DOMAIN FROM THE CLOSTRIDIUM THERMOCELLUM CELLULOSOME (MINIMIZED AVERAGE STRUCTURE)

Released:
DOI: 10.2210/pdb1daq/pdb
PDB entry 1daq coloured by chain, front view.
PDB entry 1daq coloured by chain, side view.
PDB entry 1daq coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain.
Lytle BL, Volkman BF, Westler WM, Heckman MP, Wu JH
J Mol Biol 307 745-53 (2001)
PMID: 11273698

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulose 1,4-beta-cellobiosidase (reducing end) CelS Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2S5 (Residues: 672-741; Coverage: 10%)
Gene name: celS
Sequence domains: Dockerin type I domain
Structure domains: Dockerin domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides.
Bayer et al. (2004)
7 more

4 mentions without citation

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex.
Carvalho et al. (2003)
3 more