› 1d2h

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF R175K MUTANT GLYCINE N-METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLHOMOCYSTEINE

Released:
DOI: 10.2210/pdb1d2h/pdb
PDB entry 1d2h coloured by chain, front view.
PDB entry 1d2h coloured by chain, side view.
PDB entry 1d2h coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes.
Huang Y, Komoto J, Konishi K, Takata Y, Ogawa H, Gomi T, Fujioka M, Takusagawa F
J Mol Biol 298 149-62 (2000)
PMID: 10756111

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13255 (Residues: 2-293; Coverage: 100%)
Gene names: Fbp-cII, Gnmt
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43
Unit cell:
a: 77.9Å b: 77.9Å c: 227.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.247
Expression system: Escherichia coli

Quick links

Citations

5 review citations

AdoMet-dependent methylation, DNA methyltransferases and base flipping.
Cheng et al. (2001)
4 more

3 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
2 more