› 1czd

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF THE PROCESSIVITY CLAMP GP45 FROM BACTERIOPHAGE T4

Released:
DOI: 10.2210/pdb1czd/pdb
PDB entry 1czd coloured by chain, front view.
PDB entry 1czd coloured by chain, side view.
PDB entry 1czd coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Crystal structure of the DNA polymerase processivity factor of T4 bacteriophage.
Moarefi I, Jeruzalmi D, Turner J, O'Donnell M, Kuriyan J
J Mol Biol 296 1215-23 (2000)
PMID: 10698628

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sliding clamp Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 228 amino acids
Theoretical weight: 24.88 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04525 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 66.177Å b: 93.938Å c: 141.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.259
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Eukaryotic DNA polymerases.
Hubscher et al. (2002)
27 more

11 mentions without citation

Bacteriophage T4 genome.
Miller et al. (2003)
10 more