1cv7

X-ray diffraction
2.5Å resolution

Crystal structure of enhanced cyan-emission variant of GFP

Released:
Source organism: Aequorea victoria
Entry authors: O'Brien P, Elsliger MA, Kallio K, Remington SJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.71 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-228; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 52.052Å b: 62.781Å c: 66.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Escherichia coli