1cse

X-ray diffraction
1.2Å resolution

THE HIGH-RESOLUTION X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN SUBTILISIN CARLSBERG AND EGLIN C, AN ELASTASE INHIBITOR FROM THE LEECH HIRUDO MEDICINALIS. STRUCTURAL ANALYSIS, SUBTILISIN STRUCTURE AND INTERFACE GEOMETRY

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Keratinase Chain: E
Molecule details ›
Chain: E
Length: 274 amino acids
Theoretical weight: 27.31 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
UniProt:
  • Canonical: B0FXJ2 (Residues: 81-354; Coverage: 77%)
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Eglin C Chain: I
Molecule details ›
Chain: I
Length: 70 amino acids
Theoretical weight: 8.1 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P01051 (Residues: 1-70; Coverage: 100%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 38.3Å b: 41.5Å c: 57Å
α: 111.8° β: 85.8° γ: 104.7°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Not provided