X-ray diffraction
2Å resolution

Crystal structure of cathepsin b inhibited with CA030 at 2.1 angstroms resolution: A basis for the design of specific epoxysuccinyl inhibitors


Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cathepsin B light chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 47 amino acids
Theoretical weight: 5.21 KDa
Source organism: Homo sapiens
  • Canonical: P07858 (Residues: 80-126; Coverage: 15%)
Gene names: CPSB, CTSB
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Cathepsin B heavy chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 205 amino acids
Theoretical weight: 22.44 KDa
Source organism: Homo sapiens
  • Canonical: P07858 (Residues: 129-333; Coverage: 64%)
Gene names: CPSB, CTSB
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 86.51Å b: 34.15Å c: 85.53Å
α: 90° β: 103.1° γ: 90°
R R work R free
0.194 0.194 not available