1coe › Citations

Solution conformation of cobrotoxin: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study.

Yu C, Bhaskaran R, Chuang LC, Yang CC
Biochemistry 32 2131-6 (1993)
Cited: 26 times
EuropePMC logo PMID: 8443154

Abstract

The solution conformation of cobrotoxin has been determined by using proton nuclear magnetic resonance spectroscopy. With the combination of various two-dimensional NMR techniques, the 1H-NMR spectrum of cobrotoxin was completely assigned (Yu et al., 1990). A set of 435 approximate interproton distance restraints was derived from nuclear Overhauser enhancement (NOE) measurements. These NOE constraints, in addition to the 29 dihedral angle constraints (from coupling constant measurements) and 26 hydrogen bonding restraints (from the pattern of short-range NOEs), form the basis of 3-D structure determination by the hybrid distance geometry-dynamical simulated annealing method. The 23 structures that were obtained satisfy the experimental restraints, display small deviation from idealized covalent geometry, and possess good nonbonded contacts. Analysis of converged structures indicated that there are two antiparallel beta sheets (double and triple stranded), duly confirming our earlier observations. These are well defined in terms of both atomic root mean square (RMS) differences and backbone torsional angles. The average backbone RMS deviation between the calculated structures and the mean structure, for the beta-sheet regions, is 0.92 A. The mean solution structure was compared with the X-ray crystal structure of erabutoxin b, the homologous protein. This yielded information that both structures resemble each other except at the exposed loop/surface regions, where the solution structure seems to possess more flexibility.

Reviews - 1coe mentioned but not cited (2)

  1. Causes and Consequences of Snake Venom Variation. Casewell NR, Jackson TNW, Laustsen AH, Sunagar K. Trends Pharmacol Sci 41 570-581 (2020)
  2. Snake Venomics: Fundamentals, Recent Updates, and a Look to the Next Decade. Tan CH. Toxins (Basel) 14 247 (2022)

Articles - 1coe mentioned but not cited (1)

  1. Development of Antibody Detection ELISA Based on Immunoreactive Toxins and Toxin-Derived Peptides to Evaluate the Neutralization Potency of Equine Plasma against Naja atra in Taiwan. Liu CC, Hsiao YC, Chu LJ, Wang PJ, Liu CH, Hsieh WC, Yu JS. Toxins (Basel) 13 (2021)


Reviews citing this publication (4)

  1. Snake venom cardiotoxins-structure, dynamics, function and folding. Kumar TK, Jayaraman G, Lee CS, Arunkumar AI, Sivaraman T, Samuel D, Yu C. J Biomol Struct Dyn 15 431-463 (1997)
  2. Snake venom toxins: toxicity and medicinal applications. Chan YS, Cheung RCF, Xia L, Wong JH, Ng TB, Chan WY. Appl Microbiol Biotechnol 100 6165-6181 (2016)
  3. Toxins in anti-nociception and anti-inflammation. Rajendra W, Armugam A, Jeyaseelan K. Toxicon 44 1-17 (2004)
  4. CD59: its role in complement regulation and potential for therapeutic use. Sugita Y, Masuho Y. Immunotechnology 1 157-168 (1995)

Articles citing this publication (19)

  1. Structure of a soluble, glycosylated form of the human complement regulatory protein CD59. Fletcher CM, Harrison RA, Lachmann PJ, Neuhaus D. Structure 2 185-199 (1994)
  2. The structure of Escherichia coli heat-stable enterotoxin b by nuclear magnetic resonance and circular dichroism. Sukumar M, Rizo J, Wall M, Dreyfus LA, Kupersztoch YM, Gierasch LM. Protein Sci 4 1718-1729 (1995)
  3. Structure-function relationships of the complement regulatory protein, CD59. Petranka J, Zhao J, Norris J, Tweedy NB, Ware RE, Sims PJ, Rosse WF. Blood Cells Mol Dis 22 281-296 (1996)
  4. Sequence-specific 1H-NMR assignments and folding topology of human CD59. Fletcher CM, Harrison RA, Lachmann PJ, Neuhaus D. Protein Sci 2 2015-2027 (1993)
  5. Cloning and characterization of the pseudonajatoxin b precursor. Gong N, Armugam A, Mirtschin P, Jeyaseelan K. Biochem J 358 647-656 (2001)
  6. Structurally homologous toxins isolated from the Taiwan cobra (Naja naja atra) differ significantly in their structural stability. Sivaraman T, Kumar TK, Tu YT, Peng HJ, Yu C. Arch Biochem Biophys 363 107-115 (1999)
  7. Letter Cardiotoxin-like basic protein (CLBP) from Naja naja atra is not a cardiotoxin. Sivaraman T, Kumar TK, Yang PW, Yu C. Toxicon 35 1367-1371 (1997)
  8. Secondary structure formation is the earliest structural event in the refolding of an all beta-sheet protein. Sivaraman T, Kumar TK, Tu YT, Wang W, Lin WY, Chen HM, Yu C. Biochem Biophys Res Commun 260 284-288 (1999)
  9. Biomimetic affinity purification of cardiotoxin and its pharmacological effects on the nervous system. Dong D, Liu H, Xiao Q, Wang T, Liu H, Li R. J Mol Recognit 21 148-153 (2008)
  10. Refolding of a small all beta-sheet protein proceeds with accumulation of kinetic intermediates. Hsieh HC, Kumar TK, Sivaraman T, Yu C. Arch Biochem Biophys 447 147-154 (2006)
  11. Cloning, overexpression, and characterization of cobrotoxin. Hsieh HC, Kumar TK, Yu C. Biochem Biophys Res Commun 320 1374-1381 (2004)
  12. Induction of helical conformation in all beta-sheet proteins by trifluoroethanol. Arunkumar AI, Kumar TK, Jayaraman G, Samuel D, Yu C. J Biomol Struct Dyn 14 381-385 (1996)
  13. Enrichment of the antibodies against the C-terminus of Taiwan cobra cobrotoxin using dimeric glutaraldehyde-modified toxin as an immunogen. Chang LS, Lin R, Chen KC, Chang CC. Toxicon 41 181-186 (2003)
  14. OneG: a computational tool for predicting cryptic intermediates in the unfolding kinetics of proteins under native conditions. Richa T, Sivaraman T. PLoS One 7 e32465 (2012)
  15. Comparison of three classes of snake neurotoxins by homology modeling and computer simulation graphics. Juan HF, Hung CC, Wang KT, Chiou SH. Biochem Biophys Res Commun 257 500-510 (1999)
  16. High resolution x-ray analysis of two mutants of a curaremimetic snake toxin. Gaucher JF, Ménez R, Arnoux B, Pusset J, Ducruix A. Eur J Biochem 267 1323-1329 (2000)
  17. The Three-Dimensional NMR Solution Structure of α-Cobratoxin at pH 7.5 and Conformational Differences With the NMR Solution Structure at pH 3.2. Leroy E, Mikou A, Yang Y, Guittet E. J Biomol Struct Dyn 12 1-17 (1994)
  18. Detection of Naja atra Cardiotoxin Using Adenosine-Based Molecular Beacon. Shi YJ, Chen YJ, Hu WP, Chang LS. Toxins (Basel) 9 (2017)
  19. Congress 10th International Conference on Methods in Protein Structure Analysis. September 8-13, 1994, Snowbird, Utah. Short communications and abstracts. J Protein Chem 13 431-543 (1994)


Quick links