1cnu

X-ray diffraction
2.25Å resolution

PHOSPHORYLATED ACTOPHORIN FROM ACANTAMOEBA POLYPHAGA

Released:
Source organism: Acanthamoeba polyphaga
Primary publication:
Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin.
J. Biol. Chem. 273 25106-11 (1998)
PMID: 9737968

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actophorin Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.52 KDa
Source organism: Acanthamoeba polyphaga
Expression system: Escherichia coli
UniProt:
  • Canonical: P37167 (Residues: 2-138; Coverage: 99%)
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: P212121
Unit cell:
a: 40.2Å b: 46.5Å c: 68.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.304
Expression system: Escherichia coli