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CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION

X-ray diffraction
2.7Å resolution
PDB entry 1clq coloured by chain, front view.
PDB entry 1clq coloured by chain, side view.
PDB entry 1clq coloured by chain, top view.
Source organism: Escherichia phage RB69
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-directed DNA polymerase and DNA
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Multimeric state: hetero trimer
Accessible surface area: 45515.46 Å2
Buried surface area: 5086.74 Å2
Dissociation area: 910.6 Å2
Dissociation energy (ΔGdiss): 13.64 kcal/mol
Dissociation entropy (TΔSdiss): 10.11 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113547

Macromolecules

DNA-directed DNA polymerase
Chain: A
Length: 903 amino acids
Theoretical weight: 104.74 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Pfam:
InterPro:
CATH:
SCOP:
DNA-directed DNA polymerase in PDB entry 1clq, assembly 1, front view.
DNA-directed DNA polymerase in PDB entry 1clq, assembly 1, side view.
DNA-directed DNA polymerase in PDB entry 1clq, assembly 1, top view.
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DNA chain E
Name: DNA (5'-D(*GP*CP*GP*GP*AP*AP*CP*TP*AP*CP*T)-3')
Representative chains: E
Source organism: Escherichia phage RB69 [12353]
Expression system: Not provided
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
DNA (5'-D(*GP*CP*GP*GP*AP*AP*CP*TP*AP*CP*T)-3') in PDB entry 1clq, assembly 1, front view.
DNA (5'-D(*GP*CP*GP*GP*AP*AP*CP*TP*AP*CP*T)-3') in PDB entry 1clq, assembly 1, side view.
DNA (5'-D(*GP*CP*GP*GP*AP*AP*CP*TP*AP*CP*T)-3') in PDB entry 1clq, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*AP*GP*TP*AP*GP*TP*TP*CP*CP*GP*CP*G)-3')
Representative chains: D
Source organism: Escherichia phage RB69 [12353]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
DNA (5'-D(*AP*GP*TP*AP*GP*TP*TP*CP*CP*GP*CP*G)-3') in PDB entry 1clq, assembly 1, front view.
DNA (5'-D(*AP*GP*TP*AP*GP*TP*TP*CP*CP*GP*CP*G)-3') in PDB entry 1clq, assembly 1, side view.
DNA (5'-D(*AP*GP*TP*AP*GP*TP*TP*CP*CP*GP*CP*G)-3') in PDB entry 1clq, assembly 1, top view.
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