1clq

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 903 amino acids
Theoretical weight: 104.74 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*GP*AP*AP*CP*TP*AP*CP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*AP*GP*TP*TP*CP*CP*GP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R3
Unit cell:
a: 209.74Å b: 209.74Å c: 114.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.284
Expression systems:
  • Escherichia coli
  • Not provided