1cl0

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.

Released:

Function and Biology Details

Reaction catalysed:
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9P4 (Residues: 2-321; Coverage: 100%)
Gene names: JW0871, b0888, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6322
Unit cell:
a: 121.298Å b: 121.298Å c: 81.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.292
Expression system: Escherichia coli