› 1cgv

X-ray diffraction
2.5Å resolution

SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY

Released:
DOI: 10.2210/pdb1cgv/pdb
PDB entry 1cgv coloured by chain, front view.
PDB entry 1cgv coloured by chain, side view.
PDB entry 1cgv coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Penninga D, Strokopytov B, Rozeboom HJ, Lawson CL, Dijkstra BW, Bergsma J, Dijkhuizen L
Biochemistry 34 3368-76 (1995)
PMID: 7880832

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.56 KDa
Source organism: Niallia circulans
Expression system: Not provided
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 121.031Å b: 111.107Å c: 66.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 not available not available
Expression system: Not provided

Quick links

Citations

11 review citations

Protein engineering of bacterial alpha-amylases.
Nielsen et al. (2000)
10 more

1 mention without citation

Local functional descriptors for surface comparison based binding prediction.
Cipriano et al. (2012)